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Reporter Gene Imaging. Using antibody fragments as artificial receptors Ligands Antibody fragments...

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Reporter Gene Imaging
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Page 1: Reporter Gene Imaging. Using antibody fragments as artificial receptors Ligands Antibody fragments Membrane anchoring domains Hapten-X* Hapten 1 -X*Hapten.

Reporter Gene Imaging

Page 2: Reporter Gene Imaging. Using antibody fragments as artificial receptors Ligands Antibody fragments Membrane anchoring domains Hapten-X* Hapten 1 -X*Hapten.

Using antibody fragments as artificial “receptors”

Ligands

Antibody fragments

Membraneanchoringdomains

Hapten

-X*

Hapten

1-X

*

Hapten

2-Y

*

AB

Vector

mRNA

DNA

mRNA mRNA

tissuespecificpromoter

Constitutivepromoter

Page 3: Reporter Gene Imaging. Using antibody fragments as artificial receptors Ligands Antibody fragments Membrane anchoring domains Hapten-X* Hapten 1 -X*Hapten.

Antibody reporter DAbR1

U87-DAbR1

0

10

20

30

40

50

60

HSVtk/FHBG

Reporter Gene / Reporter Probe

%ID

/g

tumor+ tumor-

blood

DAbR1/AABD

Liver

PET/CT with Y86-DOTAComparison with HSVtk reporter

Page 4: Reporter Gene Imaging. Using antibody fragments as artificial receptors Ligands Antibody fragments Membrane anchoring domains Hapten-X* Hapten 1 -X*Hapten.

Translational Molecular Imaging

Hohe Sensitifität (pmol/l)Nachweis von RezeptorenMöglich

Minimalste Stoffmengen!Keine pharmakologischenEffekte

Page 5: Reporter Gene Imaging. Using antibody fragments as artificial receptors Ligands Antibody fragments Membrane anchoring domains Hapten-X* Hapten 1 -X*Hapten.

Untersuchung der myokardialen Perfusion und des Sauerstoffverbrauchs mit 11C-1-acetate

Ng et al. Am J Physiol 1994

Page 6: Reporter Gene Imaging. Using antibody fragments as artificial receptors Ligands Antibody fragments Membrane anchoring domains Hapten-X* Hapten 1 -X*Hapten.

Sun et al. J Nucl Med 1998

Untersuchung der myokardialen Perfusion und des Sauerstoffverbrauchs mit 11C-1-acetate

Page 7: Reporter Gene Imaging. Using antibody fragments as artificial receptors Ligands Antibody fragments Membrane anchoring domains Hapten-X* Hapten 1 -X*Hapten.

Bengel et al.EJNM 2000

Untersuchung der myokardialen Effizienz mit 11C-1-acetate

Page 8: Reporter Gene Imaging. Using antibody fragments as artificial receptors Ligands Antibody fragments Membrane anchoring domains Hapten-X* Hapten 1 -X*Hapten.

Multimodale PET/CT Bildgebung

LAD Stenose ohne Ischämie

Page 9: Reporter Gene Imaging. Using antibody fragments as artificial receptors Ligands Antibody fragments Membrane anchoring domains Hapten-X* Hapten 1 -X*Hapten.

Multimodale PET/CT Bildgebung

LCX Stenose mit ausgeprägter Ischämie


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